Sulfanilamide Experiments: to investigate the effectiveness of sulfanilamide. Wounds deliberately inflicted on the victims were infected with bacteria such as streptococcus, gas gangrene, and tetanus. Circulation of blood was interrupted by tying off blood vessels at both ends of the wound to create a condition similar to that of a battlefield wound. Infection was aggravated by forcing wood shavings and ground glass into the wounds. The infection was treated with sulfanilamide and other drugs to determine their effectiveness. Many victims died as a result of these experiments and others suffered serious injury and intense agony.